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Effects of Intermittent Hypoxia on Expression of Glucose Metabolism Genes in MCF7 Breast Cancer Cell Line

[ Vol. 20 , Issue. 3 ]

Author(s):

Yazun Jarrar, Malek Zihlif*, Abdel Qader Al Bawab and Ahmed SharabPages 216-222 (7)

Abstract:


Background: Hypoxic condition induces molecular alterations which affect the survival rate and chemo-resistant phenotype of cancer cells.

Objective: This study aimed to investigate the influence of intermittent hypoxic condition on the expression of glucose metabolism genes in breast cancer MCF7 cell line.

Methods: The gene expression was analyzed using polymerase chain reaction- array method. In addition, the cell resistance, survival and migration rates were examined; to assure the hypoxic influence on the cells.

Results: We found that 30 hypoxic episodes induced Warburg effect through significant (p value < 0.05) upregulation the expression of PCK2, PHKG1, ALDOC, G6PC, GYS2, ALDOB, HK3, PKLR, PGK2, PDK2, ACO1 and H6PD genes that are involved in glycolysis. Furthermore, the expression of major gluconeogenesis enzyme genes was significantly (ANOVA, p value < 0.05) downregulated. These molecular alterations were associated with increased MCF7 cell division and migration rate. However, molecular and phenotypic changes induced after 30 episodes were normalized in MCF7 cells exposed to 60 hypoxic episodes.

Conclusion: It is concluded, from this study, that 30 intermitted hypoxic episodes increased the survival rate of MCF7 breast cancer cells and induced Warburg effect through upregulation the expression of genes involved in the glycolysis pathway. These results may increase our understanding of the molecular alterations of breast cancer cells under hypoxic condition.

Keywords:

Hypoxia, MCF7 cells, glycolysis, warburg, cell resistance, glycolysis pathway.

Affiliation:

Department of Pharmaceutical Science, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Department of Pharmacology, Faculty of Medicine, University of Jordan, Amman, Department of Pharmaceutical Science, Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman, Department of Pharmacology, Faculty of Medicine, University of Jordan, Amman



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